Tuesday 7 September 1965 at Millmoor (Att 11,370)

Rotherham United (1) 4 (Pring 20, Lyons 47, Williams 59 75): Morritt, Wilcoxson, Clish, Hardy, Madden, Tiler, Lyons, Chappell, Galley, Williams, Pring; Sub Casper.
Wolves (0) 3 (Wagstaffe 49, Knowles 72, Wharton 84p): MacLaren, J Wilson, Thomson, Flowers, Woodfield, Miller, Wharton, Woodruff, McIlmoyle, Knowles, Wagstaffe; Sub Holsgrove.

Referee: Mr R Egan (Lymm, Cheshire).
Order of Goals: r,r,W,r,W,r,W.
Penalty: Awarded for handball against ex-Wolves forward John Galley.
Position: 13th.